Degradation and cell culture studies on block copolymers prepared by ring opening polymerization of ϵ‐caprolactone in the presence of poly(ethylene glycol)

• DOI: 10.1002/jbm.a.30008
• Publication Date: 2004-04-15T19:04:10Z
• Authors: Ming‐Hsi Huang; Suming Li; Dietmar Werner Hu...; Jan‐Thorsten Schantz; Charles Alfred Va...; Christian Braud; Michel Vert
• Keywords: [CHIM.POLY] Chemical Sciences/Polymers; Hydrolytic degradation; Cell Culture Techniques; 610; Biocompatible Materials; Bone Marrow Cells; 02 engineering and technology; Polyethylene Glycols; Lactones; Poly(ε-caprolactone); Animals; Humans; Tissue engineering; hydrolytic degradation; Caproates; poly(e-caprolactone); Cells, Cultured; Microscopy; poly(ethylene glycol); Poly(ethylene glycol); Bone marrow stromal cells; 540; Rats; [CHIM.POLY]Chemical Sciences/Polymers; tissue engineering; Stromal Cells; 0210 nano-technology; bone marrow stromal cells

AbstractPoly(ϵ‐caprolactone) (PCL) and its block copolymers with poly(ethylene glycol) (PEG) were prepared by ring‐opening polymerization of ϵ‐caprolactone in the presence of ethylene glycol or PEG, using zinc metal as catalyst. The resulting polymers were characterized by various analytical techniques such as 1H NMR, SEC, DSC, IR, X‐ray, ESEM, and CZE. PCL/PEG copolymers with long PCL chains presented the same crystalline structure as PCL homopolymer, whereas PEG‐bearing short PCL blocks retained the crystalline structure of PEG and exhibited an amphiphilic behavior in aqueous solutions. Degradation of PCL and PCL/PEG diblock and triblock copolymers was realized in a 0.13 M, pH 7.4 phosphate buffer at 37°C. The results indicated that the copolymers exhibited higher hydrophilicity and degradability compared with the PCL homopolymer. Large amounts of PEG were released from the bulk after 60 weeks' degradation. In vitro cell culture studies were conducted on scaffolds manufactured via solid free form fabrication by using primary human and rat bone marrow derived stromal cells (hMSC, rMSC). Light, scanning electron, and confocal laser microscopy, as well as immunocytochemistry, showed cell attachment, proliferation, and extracellular matrix production on the surface, as well as inside the scaffold architecture. Copolymers showed better performance in the cell culture studies than the PCL homopolymer. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 417–427, 2004