Isolation of TiNbN wear particles from a coated metal‐on‐metal bearing: Morphological characterization and in vitro evaluation of cytotoxicity in human osteoblasts

• DOI: 10.1002/jbm.b.35357
• Publication Date: 2023-12-12T21:56:44Z
• Authors: Marie‐Luise Sellin; Anika Seyfarth‐Se...; Mahammad Aziz; Christian Fabry; Katrin Wenke; Paul Johan Høl; Ivan Rios‐Mondragon; Mihaela Roxana Ci...; Marcus Frank; Rainer Bader; Anika Jonitz‐Heincke
• Keywords: Viability assay

Abstract To improve the wear resistance of articulating metallic joint endoprostheses, surfaces can be coated with titanium niobium nitride (TiNbN). Under poor tribological conditions or malalignment, occur on these implant in situ. This study investigated biological response human osteoblasts to particles generated from TiNbN‐coated hip implants. Abrasive were a simulator according ISO 14242‐1/−2 and extracted Proteinase K. Particle characteristics evaluated by electron microscopy energy dispersive x‐ray spectroscopy (EDS), inductively coupled plasma mass spectrometry (ICP‐MS) dynamic light scattering (DLS) measurements. Human exposed different particle dilutions (1:20, 1:50, 1:100), cell viability gene expression levels osteogenic markers inflammatory mediators analyzed after 4 7 days. Using ICP‐MS, EDS, DLS measurements, ~70% identified as TiNbN, ranging 39 94 nm. The exhibited flat subangular morphology. Exposure did not influence osteoblastic differentiation capacity. Protein collagen type 1, osteoprotegerin, receptor activator nuclear factor κB ligand almost unaffected. Moreover, pro‐inflammatory via interleukins 6 8 was minor induced contact. A high number TiNbN only slightly affected osteoblasts' ability compared particles. Nevertheless, further studies should investigate role peri‐implant bone tissue, especially concerning other types.