Sclerostin antibody treatment enhances metaphyseal bone healing in rats

• DOI: 10.1002/jbmr.135
• Publication Date: 2010-05-17T18:09:35Z
• Authors: Fredrik Agholme; Xiaodong Li; Hanna Isaksson; Hua Zhu Ke; Per Aspenberg
• Keywords: Sclerostin; Cancellous bone; Long bone

Abstract Sclerostin is the product of SOST gene. Loss-of-function mutations in gene result a high-bone-mass phenotype, demonstrating that sclerostin negative regulator bone mass. Primarily expressed by osteocytes bone, reported to bind LRP5/6 receptor, thereby antagonizing canonical Wnt signaling and negatively regulating formation. We therefore investigated whether systemic administration sclerostin-neutralizing antibody would increase regeneration traumatized metaphyseal rats. Young male rats had screw inserted proximal tibia were divided into six groups given 25 mg/kg or control twice week subcutaneously for 2 4 weeks. In four groups, screws tested pull-out strength. At time euthanasia, similar also was contralateral immediately. significantly increased force almost 50% compared with controls after Also, at euthanasia showed force. Micro–computed tomography (µCT) remaining two led 30% volume fraction region surrounding screw. There general trabecular thickness cancellous bone. Thus, as measured amount its mechanical resistance, formation during repair but untraumatized © 2010 American Society Bone Mineral Research.