Immune Suppressive and Bone Inhibitory Effects of Prednisolone in Growing and Regenerating Zebrafish Tissues

• DOI: 10.1002/jbmr.3231
• Publication Date: 2017-08-03T19:30:18Z
• Authors: Karina Geurtzen; Aude Vernet; Andrew Freidin; Martina Rauner; Lorenz C Hofbauer; Jürgen E Schneider; Michael Brand; Franziska Knopf
• Keywords: Prednisolone

Glucocorticoids are widely used as therapeutic agents to treat immune-mediated diseases in humans because of their anti-inflammatory and immunosuppressive effects. However, glucocorticoids have various adverse effects, particular rapid pronounced bone loss associated with fractures glucocorticoid-induced osteoporosis, a common form secondary osteoporosis. In zebrafish, which increasingly study processes regeneration disease, show detrimental effects on tissue; however, the underlying cellular mechanisms incompletely understood. Here, we that treatment glucocorticoid prednisolone impacts number, activity differentiation osteoblasts, osteoclasts, immune cells during ontogenetic growth, homeostasis, zebrafish bone. Macrophage numbers reduced both larval adult tissues, correlating decreased generation myelomonocytes enhanced apoptosis these cells. contrast, osteoblasts fail proliferate, activity, undergo incomplete differentiation. addition, mitigates number recruitment osteoclasts sites fish. combination, delay growth impair regeneration. Our demonstrates many-faceted non-mammalian vertebrates helps further establish model © 2017 American Society for Bone Mineral Research.