Anchorage‐independent phosphorylation of p130Cas protects lung adenocarcinoma cells from anoikis

• DOI: 10.1002/jcb.10322
• Publication Date: 2002-11-22T15:29:21Z
• Authors: Lin Wei; Yu Yang; Xin Zhang; Qiang Yu
• Keywords: Anoikis

Abstract The regulation and function of the signaling adaptor protein p130 Cas in tumor cell anchorage‐independent survival, or anoikis resistance, were investigated human lung adenocarcinoma cells. tyrosine phosphorylation during detachment analyzed cells compared with that normal epithelial Cell trigged rapid dephosphorylation nontumorigenic anoikis‐sensitive cells, but had no effect on anoikis‐resistant Further analysis revealed total kinase activities associated are significantly higher than which ‐associated anchorage‐dependent. Analysis two known kinases FAK Src indicated altered Inhibition specifically abolished induced anoikis. Furthermore, overexpression dominant‐negative forms also apoptosis. Taken together, these data suggest mediates a survival signal from cell–matrix interaction. Alterations lead to constitutive can prevent anoikis, hence contribute anchorage independence metastasis. J. Cell. Biochem. 87: 439–449, 2002. © 2002 Wiley‐Liss, Inc.