Positive correlation between the expression of X‐chromosome RBM genes (RBMX, RBM3, RBM10) and the proapoptotic Bax gene in human breast cancer

• DOI: 10.1002/jcb.20725
• Publication Date: 2006-03-21T20:26:49Z
• Authors: Fernando Martínez...; David Agudo; Marina Pollán; Francisco Gómez‐E...; Gema Díaz‐Gil; Raul Lucas; José Schneider
• Keywords: 0301 basic medicine; Chromosomes, Human, X; Reverse Transcriptase Polymerase Chain Reaction; RNA-Binding Proteins; Apoptosis; Breast Neoplasms; Flow Cytometry; Heterogeneous-Nuclear Ribonucleoproteins; 3. Good health; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; Humans; Female; bcl-2-Associated X Protein

Abstract In a recent report, it has been postulated that the ubiquitous RBM proteins might constitute novel family of apoptosis modulators. We measured expression X‐chromosome genes ( RBMX , RBM3 and RBM10) in 122 breast cancers by means differential RT‐PCR. Using same method, we also studied apoptosis‐related Bcl‐2 Bax . Markers hormone dependence (estrogen progesterone receptors), proliferation (Ki67 DNA‐ploidy), angiogenesis (VEGF CD105), as well oncogene (c‐erb‐B2), tumor suppressor gene (p53) were analyzed. The all was significantly associated with proapoptotic P = 0.039; < 0.001; RBM10 large variant, small 0.001). Furthermore, both variants VEGF (large 0.004; 0.003). found an association borderline significance 0.05) between variant wild‐type p53. Expression finally, high tumors ≥ 20%; 0.037). seems to be interdependent significant degree (r 0.26, 0.006). From these results, X‐chromosome, through its genes, plays formerly unknown role regulation programmed cell death (apoptosis) cancer. J. Cell. Biochem. 97: 1275–1282, 2006. © 2005 Wiley‐Liss, Inc.