Effect of dual‐specificity protein phosphatase 5 on pluripotency maintenance and differentiation of mouse embryonic stem cells

• DOI: 10.1002/jcb.23244
• Publication Date: 2011-07-05T13:12:39Z
• Authors: Qi Chen; Yang Zhou; Xiaoli Zhao; Ming Zhang
• Keywords: Homeobox protein NANOG; Rex1; Dual-specificity phosphatase

The MAPK/Erk signaling pathway is considered as a key regulator of the pluripotency and differentiation embryonic stem (ES) cells, while dual-specificity protein phosphatases (DUSPs) are negative regulators MAPK. Although DUSPs potential embryogenesis regulators, their functions in regulation ES cell have not been demonstrated. present study revealed that Dusp5 was expressed mouse (mES) cells its expression correlated with undifferentiated state these cells. Exogenous enhanced mES clonogenicity suppressed by maintaining Nanog via inhibition Erk pathway. Following knockdown, Oct4 significantly attenuated activated. Additionally, EBs derived from knockdown (KDEBs) exhibited weak adherence capability, very little outgrowth, reduction number epithelial-like Gata6 (an endodermal marker) Flk1 Twist1 (mesodermal markers) inhibited KDEBs, which indicated influenced germ layers during EB development. Collectively, this suggested plays an important role maintenance may be required for