Downregulation of microRNA‐92b‐3p suppresses proliferation, migration, and invasion of gastric cancer SGC‐7901 cells by targeting Homeobox D10

• DOI: 10.1002/jcb.29005
• Publication Date: 2019-05-20T11:30:30Z
• Authors: Cheng Li; Binliang Huo; Yongheng Wang; Chong Cheng
• Keywords: Homeodomain Proteins; 0303 health sciences; Apoptosis; 3. Good health; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Cell Movement; Stomach Neoplasms; Biomarkers, Tumor; Tumor Cells, Cultured; Humans; Neoplasm Invasiveness; Cell Proliferation; Transcription Factors

To investigate the effect and mechanism of microRNA-92b-3p (miR-92b-3p) targeting Homeobox D10 (HOXD10) on proliferation, migration, invasion gastric cancer, we detected expression miR-92b-3p HOXD10 in SGC-7901 cells. The effects or invasion, matrix metalloproteinase (MMP)-2/9 cells were measured by Cell Counting Kit-8 assay, Transwell Western blot, respectively. results showed that was increased, decreased cells, compared with human normal epithelial GES-1. Functional experiments demonstrated cell MMP-2/9 significantly inhibited silencing overexpression. Moreover, a potential target gene as evidenced TargetScan software double luciferase reporter assay. In rescue experiment, knockdown HOXD10, accompanied higher MMP-2/9, could eliminate inhibitory invasion. conclusion, is highly expressed cancer interfering its might inhibit via downregulating HOXD10.