Oxygen‐mediated regulation of skeletal muscle satellite cell proliferation and adipogenesis in culture

• DOI: 10.1002/jcp.10016
• Publication Date: 2002-08-25T21:26:02Z
• Authors: Marie Csete; Jean Walikonis; Nicole Slawny; Yuewang Wei; Sheryl Korsnes; John C. Doyle; Barbara Wold
• Keywords: Cell fate determination

Abstract Major problems in stem cell biology revolve around defining the developmental potential of populations and understanding how their is maintained or progressively restricted. Oxygen (O 2 ) an obvious environmental factor which has received little attention culturing skeletal muscle progenitor cells. In this work, we examine effects O levels on potential, proliferative capacity, phenotype adult fiber population (satellite cells), lines that model multipotential embryonic paraxial mesoderm from develops. Both satellite proliferation survival mature fibers increased physiologic (6%) vs. non‐physiologic 20% used virtually all traditional culture. Six percent conditions also accelerated up‐regulation multiple MyoD family myogenic regulatory factors (MRFs). An unexpected finding was fiber‐adherent cells could assume a non‐myogenic phenotype. By criteria molecular markers gross lipid accumulation, were found to adipocyte phenotype, did so more prominently than . Selection adipogenic fate execution adipogenesis by mesenchymal dramatically higher 20 6% , decreased associated with significantly less expression regulator, PPARγ. These results suggest pathways affected are important for proliferation, fate, parent culture, may play role vivo under normal pathologic conditions. © 2001 Wiley‐Liss, Inc.