Expression of estrogen receptor β increases integrin α1 and integrin β1 levels and enhances adhesion of breast cancer cells

• DOI: 10.1002/jcp.21932
• Publication Date: 2009-09-24T17:48:07Z
• Authors: Karolina Lindberg; Anders Ström; John G. Lock; Jan‐Åke Gustafsson; Lars‐Arne Haldosén; Luisa A. Helguero
• Keywords: Estrogen receptor alpha

Abstract Estrogen effects on mammary gland development and differentiation are mediated by two receptors (ERα ERβ). Estrogen‐bound ERα induces proliferation of epithelial cancer cells, while ERβ is important for maintenance the differentiated epithelium inhibits in different cell systems. In addition, normal breast contains higher levels compared to early stage cancers, suggesting that loss could be development. Analysis ERβ−/− mice has consistently revealed reduced expression adhesion proteins. As such, a candidate modulator homeostasis metastasis. Consequently, aim this study was analyze estrogenic cells expressing ERβ. widely found but not lines, we used positive T47‐D MCF‐7 human generate with inducible expression. Furthermore, colon lines SW480 HT‐29 were also used. Integrin α1 mRNA protein increased following β1—the unique partner integrin α1—increased only at level. enhanced formation vinculin containing focal complexes actin filaments, indicating more adhesive potential. This confirmed assays where extracellular matrix proteins, mostly laminin. associated less migration. These results indicate affects clustering consequently modulates migration cells. J. Cell. Physiol. 222:156–167, 2010. © 2009 Wiley‐Liss, Inc.