Glucose stimulates intestinal epithelial crypt proliferation by modulating cellular energy metabolism

• DOI: 10.1002/jcp.26199
• Publication Date: 2017-09-19T12:36:38Z
• Authors: Weinan Zhou; Deepti Ramachandran; Abdelhak Mansouri; Megan J. Dailey
• Keywords: Carbohydrate Metabolism; Cellular metabolism

The intestinal epithelium plays an essential role in nutrient absorption, hormone release, and barrier function. Maintenance of the is driven by continuous cell renewal stem cells located crypts. amount type diet influence this process result changes size cellular make-up tissue. mechanism underlying nutrient-driven proliferation not known, but may involve a shift intracellular metabolism that allows for more nutrients to be used manufacture new cells. We hypothesized availability drives energy small epithelial crypts could contribute increases crypt proliferation. utilized primary from C57BL/6J mice study (1) effect glucose on (2) using extracellular flux analyzer real-time metabolic measurements. found increased both glycolysis, glycolytic pathway inhibitor 2-deoxy-d-glucose (2-DG) attenuated glucose-induced Glucose did enhance oxidation, increase maximum mitochondrial respiratory capacity, which activated Akt/HIF-1α signaling pathway, might at least part responsible glycolysis These results suggest high induces inducing with no change oxidation.