Methylation of CLEC14A is associated with its expression and lung adenocarcinoma progression

• DOI: 10.1002/jcp.27112
• Publication Date: 2018-09-07T14:09:28Z
• Authors: Chongyu Su; Kang Shi; Xu Cheng; Yi Han; Yunsong Li; Daping Yu; Zhidong Liu
• Keywords: Male; 0301 basic medicine; Carcinogenesis; Adenocarcinoma of Lung; Apoptosis; DNA Methylation; Xenograft Model Antitumor Assays; Gene Expression Regulation, Neoplastic; Mice; 03 medical and health sciences; A549 Cells; Cell Movement; Disease Progression; Animals; Humans; Female; Lectins, C-Type; Promoter Regions, Genetic; Cell Adhesion Molecules; Cell Proliferation

Abstract Our main objective is probing the effect of methylation CLEC14A on its expression and lung adenocarcinoma (LUAD) progression. Microarray analysis was utilized to screen out differentially downregulated genes with hypermethylation in LUAD tissues. The level measured by western blot qRT‐PCR. Methylation‐specific‐PCR performed evaluate status . 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromid (MTT) assay used check relation between cell proliferation. Cell cycle, apoptosis, migration, invasion were respectively detected flow cytometry assay, wound healing transwell assay. Tumor xenograft models established for investigating tumor formation. tissues impaired compared that adjacent tissues, promoter highly methylated LUAD. Overexpressing or inhibiting A549 LTEP‐a‐2 cells impeded duplication cells, promoted attenuated ability, arrested cycle at G0/G1 phase. Overexpression inhibited tumorigenesis nude mice. LUAD, leading downregulation acts as an antitumor role suppressing proliferation, invasion, promoting reducing tumorigenicity Thus, inhibition a novel strategy clinic treatment