Controlled vascular corrosion casting of the rabbit eye

• DOI: 10.1002/jemt.1060100104
• Publication Date: 2005-02-24T07:26:15Z
• Authors: W. H. Fahrenbach; D. R. Bacon; J. C. Morrison; E. M. Van Buskirk
• Keywords: Rabbit (cipher)

Abstract We have refined the technique of vascular corrosion casting with methacrylate to permit reproduction physiological states tone and produce sturdy castings ocular microvasculature. The method entails careful maintenance homeostasis up moment plastic perfusion, avoidance rinsing or fixation attendant anoxia, reduction viscosity resin without impairing properties resultant polymer, addition a cross‐linking agent increase strength plastic, injection at temperature pressure. This regimen reproduces normal anatomical conditions blood vessels can be used demonstrate altered tone. In all instances, second, untouched eye serves as control for unilateral manipulations. Special problems replicating vasculature are related intraocular pressure, which opposes perfusion pressure constitutes an impediment perfusion.