Ultrastructural features of tumor‐associated mast cells in parasympathetic paragangliomas (chemodectomas) of the neck

• DOI: 10.1002/jemt.24523
• Publication Date: 2024-02-21T12:49:10Z
• Authors: Irina Chekmaryova; Dmitry Kalinin; Andrey Kostin; Igor Buchwalow; Markus Tiemann; Daniel Elieh‐Ali‐...; Dmitrii Atiakshin
• Keywords: Mast (botany)

The mechanisms of the pathogenesis neck paraganglioma (PGL) and possible role mast cells (MCs) in its development metastasis are still poorly understood. We analyzed MCs' morphologic characterization, activation, properties their cytoplasmic/released granules PGLs, using light transmission electron microscopy. Paragangliomas showed a large tumor-associated MC population both connective tissue layers tumor between cells. Notably, MCs were presented by high expression specific proteases, size variation, polymorphism, variable ultrastructural phenotype granules. A massive number released surrounding degranulated while integrity membrane was maintained. Granules electron-dense with or without membrane, ranging from 0.2 to 0.8 μm diameter. plasmalemma not found at site MC-collagen fibrils contact, whereas secretome directly contacted. observed direct mediator-based interactions latter preserved when proximity. effects on microenvironment demonstrated pathogenetic progression allow application new diagnostic criteria protocols for personalized therapy. RESEARCH HIGHLIGHTS: Ultrastructural analysis reveals novel regulatory via diverse secretory pathways parasympathetic paraganglioma, including fibrous extracellular matrix remodeling microenvironment.