Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

• DOI: 10.1002/jev2.12404
• Publication Date: 2024-02-08T03:56:02Z
• Authors: Joshua A. Welsh; Deborah C. I. Gob...; Lorraine O'Driscoll; Edit I. Buzas; Cherie Blenkiron; Benedetta Bussolati; Houjian Cai; Dolores Di Vizio; Tom A. P. Driedonks; Uta Erdbrügger; Juan M. Falcon‐Perez; Qing‐Ling Fu; Andrew F. Hill; Metka Lenassi; Sai Kiang Lim; Mỹ G. Mahoney; Sujata Mohanty; Andreas Möller; Rienk Nieuwland; Takahiro Ochiya; Susmita Sahoo; Ana C. Torrecilhas; Lei Zheng; Andries Zijlstra; Sarah Abuelreich; Reem Bagabas; Paolo Bergese; Esther M. Bridges; Marco Brucale; Dylan Burger; Randy P. Carney; Emanuele Cocucci; Rossella Crescitelli; Edveena Hanser; Adrian L. Harris; Norman J. Haughey; An Hendrix; Alexander R. Ivanov; Tijana Jovanovic‐...; Nicole A. Kruh‐Ga...; Vroniqa Ku'ulei‐L...; Diego Kyburz; Cecilia Lässer; Kathleen M. Lennon; Jan Lötvall; Adam L. Maddox; Elena S. Martens‐...; Rachel R. Mizenko; Lauren A. Newman; Andrea Ridolfi; Eva Rohde; Tatu Rojalin; Andrew Rowland; Andras Saftics; Ursula S. Sandau; Julie A. Saugstad; Faezeh Shekari; Simon Swift; Dmitry Ter‐Ovanesyan; Juan P. Tosar; Zivile Useckaite; Francesco Valle; Zoltan Varga; Edwin van der Pol; Martijn J. C. van...; Marca H. M. Wauben; Ann M. Wehman; Sarah Williams; Andrea Zendrini; Alan J. Zimmerman; ; Clotilde Théry; Kenneth W. Witwer
• Keywords: Extracellular Vesicles; Extracellular vesicles; Snapshot (computer storage); Synthetic Biology

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of cell origin and change functions phenotypes other cells. These features indicate strong biomarker therapeutic potential have generated broad interest, as evidenced by steady year-on-year increase in numbers scientific publications about EVs. Important advances been made EV metrology understanding applying biology. However, hurdles remain to realising EVs domains ranging from basic biology clinical applications due challenges nomenclature, separation non-vesicular extracellular particles, characterisation functional studies. To address opportunities this rapidly evolving field, International Society for Vesicles (ISEV) updates its 'Minimal Information Studies Vesicles', which was first published 2014 then 2018 MISEV2014 MISEV2018, respectively. The goal current document, MISEV2023, is provide researchers with an updated snapshot available approaches advantages limitations production, multiple sources, including culture, body fluids solid tissues. In addition presenting latest art principles research, document also covers advanced techniques that are currently expanding boundaries field. MISEV2023 includes new sections on release uptake a brief discussion vivo study Compiling feedback ISEV expert task forces more than 1000 researchers, conveys research facilitate robust discoveries move field forward even rapidly.