Oxidoreductase activity of multifunctional monoclonal antibody B13‐DE1

• DOI: 10.1002/jmr.1136
• Publication Date: 2011-10-05T10:09:43Z
• Authors: Katrin Messerschmidt; Janine Degen; Burkhard Micheel
• Keywords: 0303 health sciences; Antibodies, Monoclonal; Antibodies, Catalytic; 3. Good health; 03 medical and health sciences; Xanthenes; Catalytic Domain; Oxazines; Sulfites; Fluorescein; Binding Sites, Antibody; Oxidoreductases; Oxidation-Reduction; Institut für Biochemie und Biologie

The monoclonal antibody B13‐DE1 binds fluorescein, several fluorescein derivatives, and three peptide mimotopes. Our results revealed that this also catalyzed the redox reaction of resazurin to resorufin, which are both structurally related fluorescein. By using sodium sulfite as a reducing agent, lowered activation energy reaction. Michaelis–Menten constant turnover number were determined 4.2 µmol/l 0.0056 s −1 , respectively. Because showed inhibited catalytic activity antibody, we assume antigen‐binding site active identical. Copyright © 2011 John Wiley & Sons, Ltd.