Desorption electrospray ionization mass spectrometry of intact bacteria

• DOI: 10.1002/jms.1250
• Publication Date: 2007-07-13T11:16:45Z
• Authors: Mohammed A. Meetani; Yong‐Seung Shin; Shaofeng Zhang; Richard Mayer; Franco Basile
• Keywords: Spectrometry, Mass, Electrospray Ionization; Bacterial Proteins; Proteome; Gram-Negative Bacteria; Gram-Positive Bacteria; 01 natural sciences; 0104 chemical sciences

Abstract Desorption electrospray ionization (DESI) mass spectrometry (MS) was used to differentiate seven bacteria species on the basis of their measured DESI‐mass spectral profile. Both gram‐positive and gram‐negative were tested included Escherichia coli, Staphyloccocus aureus, Enterococcus sp., Bordetella bronchiseptica, Bacillus thuringiensis, subtilis Salmonella typhimurium . Distinct spectra, in range 50–500 u, obtained from whole either positive or negative ion modes less than 2 mins analysis time. Positive fingerprints compared using principal components (PCA) investigate reproducibility for intraday day‐to‐day measurements method selectivity studied. Detailed study variances assay revealed that a large contribution fingerprint variation growth media preparation procedure. Specifically, experiments conducted with prepared same batch yielded highly reproducible both analyses (i.e. one over 3‐day period versus new every day period). Conclusions are drawn our findings terms strategies rapid biodetection DESI‐MS. Copyright © 2007 John Wiley & Sons, Ltd.