Combined mass spectrometric and chromatographic methods for in-depth analysis of phenolic secondary metabolites in barley leaves

• DOI: 10.1002/jms.3557
• Publication Date: 2015-02-20T12:46:53Z
• Authors: Anna Piasecka; Aneta Sawikowska; Paweł Krajewski; Piotr Kachlicki
• Keywords: Flavonoids; 0301 basic medicine; 2. Zero hunger; Spectrometry, Mass, Electrospray Ionization; 0303 health sciences; Coumaric Acids; Plant Extracts; Hordeum; 3. Good health; Plant Leaves; 03 medical and health sciences; Tandem Mass Spectrometry; 13. Climate action; Glycosides; Chromatography, High Pressure Liquid

Structural analysis via HPLC-ESI-MSn, UPLC-HESI-MS/MS and NMR reported 152 phenolic secondary metabolites in spring barley seedlings (Hordeum vulgare L.). Flavonoids with various patterns of glycosylation acylation, as well hydroxycinnamic acid glycosides, esters amides, were identified methanolic extracts from leaves nine varieties originating different regions the world. Hordatines derivatives, flavones acylated directly on aglycone, hydroxyferulic derivatives deserve special attention. Preparative chromatography enabled characterization a number compounds at trace levels 6-C-[6″-O-glycosyl]-glycosides 6-C-[2″,6″-di-O-glycosides]-glucoside structure flavones. Derivatives flavonols, quercetin isorhamnetin observed only Syrian varieties. The ultra performance liquid profiles UV-absorbing used for chemotaxonomic comparison between climatic conditions. hierarchical clustering bred lines Fertile Crescent European American indicates great diversity chemical phenotypes within species. Copyright © 2015 John Wiley & Sons, Ltd.