Rhinovirus infects primary human airway fibroblasts and induces a neutrophil chemokine and a permeability factor

• DOI: 10.1002/jmv.20315
• Publication Date: 2005-02-16T01:21:02Z
• Authors: Reena Ghildyal; Hayat Dagher; Howard Donninger; Dinesha de Silva; Xun Li; Nicholas J. Freezer; John W. Wilson; Philip G. Bardin
• Keywords: Rhinovirus

Abstract The events linking rhinovirus (RV) infection to airway symptoms are poorly understood. virus initially infects epithelium followed by a vigorous inflammatory response that may entail spread of RV from other cells in the wall. However, has fastidious growth characteristics and date reproductive primary than human not been confirmed. Airway fibroblasts adjacent contact with epithelial cells, play key role innate immune responses, participate evolution inflammation. To investigate fibroblast actions, we first determined whether could infect replicate culture lung fibroblasts. serotype 16 (RV16) was used grown tissue, replication demonstrated combination techniques. RT‐PCR show an increase transcription; confocal microscopy colocalization replicative form genome (double‐stranded RNA) RV16 proteins; infectious also recovered supernatant infected Functional consequences were next examined. neutrophil‐activating peptide‐78 (ENA‐78) mRNA protein. permeability factor vascular endothelial (VEGF) induced over similar time course. These data suggest interactions between feasible, coordinate neutrophil chemoattraction enhanced contribute responses following infections. J. Med. Virol. 75:608–615, 2005. © 2005 Wiley‐Liss, Inc.