Isolation and biological characterization of HIV-1 BG intersubtype recombinants and other genetic forms circulating in Galicia, Spain

• DOI: 10.1002/jmv.20734
• Publication Date: 2006-10-25T01:30:19Z
• Authors: Lucía Pérez-Alvarez; Mercedes Muñoz; Elena Delgado; Celia Miralles; Antonio Ocampo; Valentina García; Michael Thomson; Gerardo Contreras; Rafael Nájera
• Keywords: Isolation; Genetic Variability

The biological characteristics of HIV-1 primary isolates different recombinant forms (RFs) and non-B subtypes from Galicia, Spain, were investigated the relationships between phenotype evolution infection determined. Peripheral blood mononuclear cells (PBMCs) obtained during follow-up 32 patients infected with non-subtype B genetic forms, characterized in partial sequences pol (protease-reverse transcriptase) env V3 region: 12 (37.5%) circulating RFs (CRFs), 9 (28.1%) unique (URFs), 11(34.4%) subtypes. Primary by coculture donor PBMCs. Syncytium-inducing (SI) was examined MT2 cell line coreceptor use GHOST U87.CD4 cells. Fifty percent tissue culture infective dose (TCID50) viral based on net charge Geno2phenocoreceptor bioinformatic method Fifty-four obtained. CRF14_BG BG URFs represented largest group, being all SI/X4, independently CD4+ count, load, or duration infection. By contrast, 10 11 CRF02_AG viruses NSI/R5. prediction co-receptor concordant characterization NSI/R5 23 26 SI/X4 isolates. presence SI/X4,R5 at early stages addition to a decrease counts below 500 cells/µl 2 6 years since diagnosis observed URFs. These data contrast usual progression subtype infections, which rarely predominate J. Med. Virol. 78:1520–1528, 2006. © 2006 Wiley-Liss, Inc.