Characterization of putative Japanese encephalitis virus receptor molecules on microglial cells

• DOI: 10.1002/jmv.23248
• Publication Date: 2012-02-15T19:51:29Z
• Authors: Thananya Thongtan; Nitwara Wikan; Phitchayapak Wint...; Chutima Rattanaru...; Chantragan Srisomsap; Poonlarp Cheepsun...; Duncan R. Smith
• Keywords: Antibody-dependent enhancement; Flavivirus; Viral encephalitis; Neurotropic virus

Japanese encephalitis virus (JEV) a mosquito-borne flavivirus is major cause of viral in Asia. While the principle target cells for JEV central nervous system are believed to be neurons, microglia activated response and have been proposed act as long lasting reservoir. Viral attachment host cell first step entry process critical mediator tissue tropism. This study sought identify molecules associated with microglial cells. Virus overlay protein-binding assay (VOPBA) liquid chromatography-mass spectrometry (LC/MS/MS) identified 37/67 kDa high-affinity laminin receptor protein nucleolin potential JEV-binding proteins. These proteins were subsequently investigated contribution mouse BV-2 together other possible candidate including Hsp70, Hsp90, GRP78, CD14, CD4. In antibody mediated inhibition infection experiments, both anti-laminin anti-CD4 antibodies significantly reduced while anti-Hsp70 90 produced slight reduction. Significant (up 80%) was observed presence lipopolysaccharide (LPS) which resulted complete down-regulation CD4 moderate CD14. results suggest that multiple may mediate cells, playing role.