Glycosphingolipid composition of primary cultured human brain microvascular endothelial cells

• DOI: 10.1002/jnr.20228
• Publication Date: 2004-08-23T19:38:02Z
• Authors: Takashi Kanda; Toshio Ariga; Hisako Kubodera; Hong Lian Jin; Kiyoshi Owada; Takeshi Kasama; Masanaga Yamawaki; Hidehiro Mizusawa
• Keywords: Glycosphingolipid; Globotriaosylceramide; Human brain

Abstract Glycosphingolipid (GSL) antigens have been considered to be involved in the pathogenesis of autoimmune neurologic disorders including multiple sclerosis. To establish GSL pattern specific for endothelial cells forming blood–brain barrier (BBB), we established a method yield sufficient quantities highly purified human brain microvascular (HBMECs) and compared their composition that umbilical cord vein (HUVECs), as representative not BBB. The major gangliosides were GM3 sialyl paragloboside (LM1), neutral GSLs lactosylceramide (LacCer), globotriaosylceramide (Gb3), globoside (Gb4). Trace amounts GM1, GD1a, GD1b, GT1b, sulfoglucuronosyl (SGPG) could detected by high performance thin layer chromatography‐overlay method. SGPG was only at nonconfluent state an amount almost 1/30 HUVECs. Conversely, LM1 increased significantly after confluency. Gb3 HBMECs twice significance these differences content between HUVECs confluent states is obscure. It might related, however, defense mechanism BBB susceptibility central nervous system some target cell surface GSL, such hemolytic uremic syndrome. © 2004 Wiley‐Liss, Inc.