Xeno‐free culture for generation of forebrain oligodendrocyte precursor cells from human pluripotent stem cells

• DOI: 10.1002/jnr.24413
• Publication Date: 2019-03-20T09:03:15Z
• Authors: Yulius Hermanto; Takakuni Maki; Yasushi Takagi; Susumu Miyamoto; Jun Takahashi
• Keywords: Forebrain; OLIG2; Neurosphere

Abstract Oligodendrocytes (OLs) show heterogeneous properties that depend on their location in the central nervous system (CNS). In this regard, investigation of oligodendrocyte precursor cells (OPCs) derived from human pluripotent stem (hPSCs) should be reconsidered, particularly cases brain‐predominant disorders for which brain‐derived OPCs are more appropriate than spinal cord‐derived OPCs. Furthermore, animal‐derived components responsible culture variability derivation and complicate clinical translation. present study, we established a xeno‐free to induce forebrain hPSCs. We induced neural (NSCs) Laminin 511‐E8 directed differentiation developmental pathway OLs with SHH FGF signaling. were characterized by expression OLIG2, NKX2.2, SOX10, PDGFRA, subsequent maturation into O4 + cells. vitro characterization showed >85% (O4 ) underwent (MBP 3 weeks after mitogen removal. Upon intracranial transplantation, survived, dispersed corpus callosum, matured (GSTπ host brains months transplantation. These findings suggest our induction hPSCs could accelerate translation brain‐specific disorders.