RECK expression in osteosarcoma: correlation with matrix metalloproteinases activation and tumor invasiveness

• DOI: 10.1002/jor.20323
• Publication Date: 2007-02-02T19:21:47Z
• Authors: Hyun‐Guy Kang; Han‐Soo Kim; Kap‐Jung Kim; Joo Han Oh; Mi‐Ra Lee; So Mi Seol; Ilkyu Han
• Keywords: Zymography

Abstract Osteosarcoma is a malignant tumor of bone characterized by its high metastatic potential. For the development metastasis, activation matrix metalloproteinases (MMPs) required. A novel MMPs inhibitor, reversion inducing cysteine rich protein with Kazal motifs (RECK), known to down‐regulate and suppress invasive potential in many tumor‐derived cell lines some types tumors. The expression RECK role invasiveness have never been studied osteosarcoma. We examined mRNA osteosarcoma using quantitative real time PCR, gelatin zymography, invasion assay, transfection experiments. was expressed but down‐regulated cells. Activation pro‐MMP‐2 observed all samples, whereas MMP‐2 pro‐MMP‐9 detected only 11% 7% respectively. MMP‐9 not activated any samples. level inversely correlated activation, overexpression resulted decreased reduced invasiveness. These findings suggest that plays an important © 2007 Orthopaedic Research Society. Published Wiley Periodicals, Inc. J Orthop Res 25:696–702,