Human peripheral blood derived mesenchymal stem cells demonstrate similar characteristics and chondrogenic differentiation potential to bone marrow derived mesenchymal stem cells

• DOI: 10.1002/jor.21556
• Publication Date: 2011-09-16T04:34:24Z
• Authors: Pan‐Pan Chong; Lakshmi Selvaratnam; Azlina A. Abbas; Tunku Kamarul
• Keywords: Chondrogenesis

Abstract The use of mesenchymal stem cells (MSCs) for cartilage repair has generated much interest owing to their multipotentiality. However, significant presence in peripheral blood (PB) been a matter debate. objectives this study are isolate and characterize MSCs derived from PB and, compare chondrogenic potential MSC bone marrow (BM). BM 20 patients were isolated characterized. From 2 ml BM, 5.4 ± 0.6 million 10.5 0.8 adherent cells, respectively, obtained by cell cultures at passage 2. Both able undergo tri‐lineage differentiation showed negative expression CD34 CD45, but positively expressed CD105, CD166, CD29. Qualitative quantitative examinations on the similar specific gene ( COMP ) proteoglycan levels, respectively. Furthermore, s‐GAG levels that native chondrocytes. In conclusion, demonstrates maintain characteristics have those while producing comparable expressions © 2011 Orthopaedic Research Society. Society Published Wiley Periodicals, Inc. J Orthop Res 30:634–642, 2012