MicroRNA‐1 (miR‐1) inhibits chordoma cell migration and invasion by targeting slug

• DOI: 10.1002/jor.22632
• Publication Date: 2014-04-24T09:01:41Z
• Authors: Eiji Osaka; Xiaoqian Yang; Jacson K. Shen; Pei Yang; Yong Feng; Henry J. Mankin; Francis J. Hornicek; Zhenfeng Duan
• Keywords: Slug; Chordoma

ABSTRACT Recent studies have revealed that expression of miRNA‐1 (miR‐1) is frequently down‐regulated in several cancer types including chordoma. Identifying and validating novel targets miR‐1 useful for understanding the roles We aimed to further investigate functions Specifically, we assessed whether restoration affects cell migration invasion chordoma, focused on potential target Slug gene. Migratory invasive activities were by wound healing Matrigel assays, respectively. Cell proliferation was determined MTT assay. evaluated Western blot, immunofluorescence, immunohistochemistry. Restoration suppressed migratory chordoma cells. Transfection inhibited both time‐ dose‐dependently MiR‐1 transfected cells showed expression. over‐expressed lines advanced tissues. In conclusion, shown directly gene not only proliferation, but also activities, reduced These results collectively indicate miR‐1/Slug pathway a therapeutic because its crucial growth migration. © 2014 Orthopaedic Research Society. Published Wiley Periodicals, Inc. J Orthop Res 32:1075–1082, 2014.