YAP and ERK mediated mechanical strain‐induced cell cycle progression through RhoA and cytoskeletal dynamics in rat growth plate chondrocytes

• DOI: 10.1002/jor.23138
• Publication Date: 2015-12-22T23:19:08Z
• Authors: Kaixiang Yang; Yingxing Wu; Peng Cheng; Jinming Zhang; Chengyuan Yang; Bin Pi; Yaping Ye; Hongbo You; Anmin Chen; Tao Xu; Fengjing Guo; Jun Qi
• Keywords: Strain (injury); Mechanotransduction; Dynamics

Yes-associated protein (YAP) and extracellular signal-regulated kinase (ERK) have been considered as key regulators in tissue homeostasis, organ development, tumor formation. However, the roles of YAP ERK mediating strain mechanosensing growth plate cartilage not determined. In this study, chondrocytes obtained from 2-week-old Sprague-Dawley rats were subjected to mechanical with different magnitudes durations at a frequency 0.5 Hz. We found that activation response was time magnitude dependent. Pretreatment RhoA inhibitor (C3 toxin) or microfilament cytoskeleton disrupting reagent (cytochalasin D) could suppress their activation. addition, activated able induce cell cycle progression by up-regulating expression cycle-related genes. These results shed new light on function strain-promoted development. Our also provided evidence cytoskeletal dynamics are required for mechanotransduction. © 2015 Orthopaedic Research Society. Published Wiley Periodicals, Inc. J Orthop Res 34:1121-1129, 2016.