Immune modulation with primed mesenchymal stem cells delivered via biodegradable scaffold to repair an Achilles tendon segmental defect

• DOI: 10.1002/jor.23258
• Publication Date: 2016-04-10T16:10:39Z
• Authors: Erdem Aktas; Connie S. Chamber...; Erin E. Saether; Sarah E. Duenwald...; Jaclyn Kondratko‐...; Michael Stitgen; Jae Sung Lee; Anna E. Clements; William L. Murphy; Ray Vanderby
• Keywords: Immune Modulation; Modulation (music)

Tendon healing is a complex coordinated series of events resulting in protracted recovery, limited regeneration, and scar formation. Mesenchymal stem cell (MSC) therapy has shown promise as new technology to enhance soft tissue bone healing. A challenge with MSC involves the ability consistently control inflammatory response subsequent Previous studies suggest that preconditioning MSCs cytokines, such IFN-γ, TNF-α, IL-1β may accelerate cutaneous wound closure. The objective this study was therefore elucidate these effects tendon. That is, vivo TNF-α primed were studied using rat Achilles segmental defect model. Rat tendons subjected unilateral 3 mm repaired either PLG scaffold alone, MSC-seeded scaffold, or TNF-α-primed scaffold. analyzed at 2 4 weeks post-injury. In vivo, MSCs, regardless priming, increased IL-10 production reduced factor, IL-1α. Primed IL-12 number M1 macrophages, well percent M2 synthesis anti-inflammatory factor IL-4. treatment also concentration type I procollagen failure stress tendon Taken together delivery via 3D modulated macrophage polarization cytokine further accentuate more regenerative MSC-induced response. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:269-280, 2017.