Effects of joint immobilization on changes in myofibroblasts and collagen in the rat knee contracture model

• DOI: 10.1002/jor.23498
• Publication Date: 2016-12-05T10:59:20Z
• Authors: Ryo Sasabe; Junya Sakamoto; Kyo Goto; Yuichiro Honda; Hideki Kataoka; Jiro Nakano; Tomoki Origuchi; Daisuke Endo; Takehiko Koji; Minoru Okita
• Keywords: Myofibroblast; Joint Contracture; Collagen fiber; Rat model

The purpose of this study was to examine the time-dependent changes in development joint capsule fibrosis and number myofibroblasts after immobilization, using a rat knee contracture model. Both joints were fixed full flexion for 1, 2, 4 weeks (immobilization group). Untreated rats bred each immobilization period (control Histological analysis performed evaluate amount density collagen capsule. type I III mRNA examined by situ hybridization. assessed immunohistochemical methods. In group, increased within 1 week 2 weeks, as compared with that control group. Type mRNA-positive cell numbers group at all time points. However, did not increase. Myofibroblasts significantly those points, they immobilization. These results suggest overexpression occurs progresses an increase is related mechanism fibrosis. findings need treatment targeting accumulation associated myofibroblasts. © 2017 Orthopaedic Research Society. Published Wiley Periodicals, Inc. J Orthop Res 35:1998–2006, 2017.