Comparison of High‐Hydrostatic‐Pressure Decellularized Versus Freeze‐Thawed Porcine Menisci

• DOI: 10.1002/jor.24350
• Publication Date: 2019-05-22T09:53:20Z
• Authors: Naoto Watanabe; Mitsuru Mizuno; Junpei Matsuda; Naoko Nakamura; Koji Otabe; Hisako Katano; Nobutake Ozeki; Yuji Kohno; Tsuyoshi Kimura; Kunikazu Tsuji; Hideyuki Koga; Akio Kishida; Ichiro Sekiya
• Keywords: Decellularization; Meniscus; Hydrostatic pressure

The meniscus functions as a load distributor and secondary stabilizer in the knee, loss of increases risk osteoarthritis. Freeze-thawed menisci are used clinical practice to replace defective menisci; however, disadvantages freeze-thawed tissues include disease transmission immune rejection. In this study, we decellularized using high hydrostatic pressure (HHP) compared with menisci. Porcine were either pressurized at 1,000 MPa for 10 min then washed DNase solution or frozen -80°C 2 days thawed. These underwent vitro histological, biochemical, biomechanical comparisons native HHP-treated also subcutaneously implanted pig, later harvested histological analysis. numbers histologically detected cells significantly lower amount biochemically DNA was approximately 100-fold than compression strength HHP-decellularized decreased after 1 50 cycles 20% strain but unchanged After implantation, multinucleated giant around Recellularization confirmed. Thus, although mechanically inferior vitro, they immunologically superior. Our study is first demonstrate use HHP decellularization meniscus. © 2019 Authors. Journal Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf Research Society. J Orthop Res 37:2466-2475, 2019.