Implementation of a novel low‐noise InGaAs detector enabling rapid near‐infrared multichannel Raman spectroscopy of pigmented biological samples

• DOI: 10.1002/jrs.4714
• Publication Date: 2015-05-14T09:11:26Z
• Authors: Inês P. Santos; Peter J. Caspers; Tom Bakker Schut; Remco van Doorn; Senada Koljenović; Gerwin J. Puppels
• Keywords: noise; InGaAs; EMC MM-03-61-05-A; pigmented; 0103 physical sciences; fluorescence; EMC MM-03-24-01; 01 natural sciences; SWIR

Pigmented tissues are inaccessible to Raman spectroscopy using visible laser light because of the high level laser‐induced tissue fluorescence. The fluorescence contribution acquired signal can be reduced by an excitation wavelength in near infrared range around 1000 nm. This will shift spectrum above 1100 nm, which is principal upper detection limit for silicon‐based CCD detectors. For wavelengths nm indium gallium arsenide detectors used. However, InGaAs have not yet demonstrated satisfactory noise characteristics demanding applications. We tested and implemented first time a novel sensitive imaging camera with extremely low readout multichannel short‐wave (SWIR) region. effective two electrons comparable that quality CCDs orders magnitude lower than other commercially available detector arrays. With in‐house built system we demonstrate shot‐noise limited spectra pigmented samples wavenumber region, whereas more traditional (671 nm) could generate useful Our instrument makes it possible substantially decrease background obtain from biological integration times well below 20 s. Copyright © 2015 John Wiley & Sons, Ltd.