Estimation of the number of Anisakis larvae in commercial fish using a descriptive model based on real‐time PCR

• DOI: 10.1002/jsfa.10718
• Publication Date: 2020-08-08T13:51:55Z
• Authors: Carla Godínez‐Gon...; Xavier Roca‐Geronès; Isabel Montoliu; Roser Fisa
• Keywords: Anisakidae; Anisakis; Anisakis simplex

Abstract BACKGROUND Seafood parasitation by Anisakis (Anisakidae) larvae has been reported in most of the oceans and seas worldwide. The presence these nematodes commonly consumed fish represents a potential hazard for consumers as they can provoke gastrointestinal symptoms allergic reactions. In present work, capacity SYBR Green qPCR protocol to quantify commercial was evaluated using experimentally spiked samples with different numbers (0–50) A. simplex third‐stage (L3). To verify agreement obtained results, 25 naturally infected specimens Atlantic blue whiting underwent parallel visual inspection. RESULTS logarithmic behavior Cq data from allowed development descriptive mathematical model that correlates value number ( R 2 = 0.9908, CV 2.37%). there high correlation between results molecular technique inspection 0.9912); Bland–Altman analysis showed 94% differences were within limits (−4.98 6.68), indicating reliability based on technique. CONCLUSION function presented assay is promising sensitive accurate tool measuring larval load fish, application not only food industry but also prevention programs public health. © 2020 Society Chemical Industry