Conversion of intratumoral regulatory T cells by human gastric cancer cells is dependent on transforming growth factor‐β1

• DOI: 10.1002/jso.22005
• Publication Date: 2011-06-21T15:41:16Z
• Authors: Xiaoming Lu; Jun Liu; Hang Li; Wei Li; Xuesong Wang; Jingwei Ma; Qiang Tong; Ke Wu; Guobin Wang
• Keywords: Regulatory T cell

Abstract Background and Objectives Regulatory T cells (Treg) inhibits immune responses mediated by cells. This study aimed to investigate whether Treg are accumulated in human gastric cancer tissue the mechanism of induction Methods Tissue infiltrated leukocytes from adenocarcinomas were subjected flow cytometry immunohistochemistry. Percentage, phenotype, function, clinical relevance analyzed. TGF‐β1 production was determined Western blot vitro co‐culture experiments performed mimic microenvironment. Results The percentages CD4 + Foxp3 tissues significantly higher than those adjacent non‐tumor ( P < 0.05). results classical phenotype proliferation assay supported that elevated represents a suppressive population. High proportion is correlated advance TNM stage reduced survival. Primary produced abundance which responsible for conversion Treg. Conclusion functional related poor prognosis. Gastric directly convert naive TGF‐β1, suggesting possible through tumor evade system. J. Surg. Oncol. 2011; 104:571–577. © 2011 Wiley Periodicals, Inc.