Dual Photothermal/Chemotherapy of Melanoma Cells with Albumin Nanoparticles Carrying Indocyanine Green and Doxorubicin Leads to Immunogenic Cell Death

• DOI: 10.1002/mabi.202100353
• Publication Date: 2021-11-11T16:25:02Z
• Authors: Niloofar Heshmati...; Susana Torres Hur...; Shahad M. Abdulsahib; Emilio J. Lara; James W. Tunnell; Tania Betancourt
• Keywords: Indocyanine Green; Immunogenic cell death; Bovine serum albumin

Recent focus on cancer immunotherapies has led to significant interest in the development of therapeutic strategies that can lead immunogenic cell death (ICD), which cause activation an immune response against tumor cells and improve immunotherapy outcomes by enhancing immunogenicity microenvironment. In this work, a nanomedicine-mediated combination therapy is used deliver ICD inducers doxorubicin (Dox), chemotherapeutic agent, indocyanine green (ICG), photothermal agent. These agents are loaded into nanoparticles (NPs) bovine serum albumin (BSA) prepared through desolvation process. The formulation BSA NPs optimized achieve 102.6 nm size loadings 8.55 % 5.69 (w/w) for ICG Dox, respectively. controlled release these from confirmed. Upon laser irradiation 2.5 min, at dose 62.5 μg mL-1 able increase temperature 7 °C thereby inhibit growth B16F10 melanoma vitro. Surface presentation heat shock proteins calreticulin after treatment confirmed ability Dox/ICG induce cells.