Drop‐On‐Drop Multimaterial 3D Bioprinting Realized by Peroxidase‐Mediated Cross‐Linking

• DOI: 10.1002/marc.201700534
• Publication Date: 2017-12-11T06:12:50Z
• Authors: Shinji Sakai; Kohei Ueda; Enkhtuul Gantumur; Masahito Taya; Makoto Nakamura
• Keywords: Biofabrication; Horseradish peroxidase; Gelatin

Abstract A cytocompatible inkjet bioprinting approach that enables the use of a variety bioinks to produce hydrogels with wide range characteristics is developed. Stabilization caused by horseradish peroxidase (HRP)‐catalyzed cross‐linking consuming hydrogen peroxide (H 2 O ). 3D cell‐laden are fabricated sequential dropping bioink containing polymer(s) cross‐linkable through enzymatic reaction and H onto droplets another polymer, HRP, cells. The ≈95% viability enclosed mouse fibroblasts subsequent elongation cells in bioprinted hydrogel consisting gelatin hyaluronic acid derivatives suggest high cytocompatibility developed printing approach. existence numerous polymers, including polysaccharides, proteins, synthetic HRP‐catalyzed reaction, means current shows great promise for biofabrication functional structurally complex tissues.