G protein‐coupled estrogen receptor agonist G‐1 decreases ADAM10 levels and NLRP3‐inflammasome component activation in response to Staphylococcus aureus alpha‐hemolysin

• DOI: 10.1002/mbo3.1423
• Publication Date: 2024-06-13T05:09:43Z
• Authors: Huayu Zheng; Kathleen D. Triplett; Eric R. Prossnitz; Pamela R. Hall; Seth M. Daly
• Keywords: GPER; ADAM10

Abstract The G protein‐coupled estrogen receptor, also known as GPER1 or originally GPR30, is found in various tissues, indicating its diverse functions. It typically present immune cells, suggesting role regulating responses to infectious diseases. Our previous studies have shown that G‐1, a selective GPER agonist, can limit the pathogenesis mediated by Staphylococcus aureus alpha‐hemolysin (Hla). aids clearing bacteria mouse skin infection model and restricts surface display of Hla ADAM10 (a disintegrin metalloprotease 10) HaCaT keratinocytes. In this report, we delve into modulation human cells relation NLRP3 inflammasome. We used macrophage‐like differentiated THP‐1 for our study. treating these with G‐1 reduces ATP release, decreases activity caspase‐1 enzyme, lessens cell death following intoxication. This likely due reduced levels proteins, well decreased receptor G‐1‐treated cells. studies, along work, suggest potential therapeutic use reducing susceptibility humans. highlights importance regulation target.