Agarolytic bacteriumPersicobactersp. CCB-QB2 exhibited a diauxic growth involving galactose utilization pathway

• DOI: 10.1002/mbo3.405
• Publication Date: 2016-12-17T17:21:03Z
• Authors: Go Furusawa; Nyok-Sean Lau; Appalasamy Suganthi; Abdullah Al-Ashra...
• Keywords: DNA, Bacterial; 0303 health sciences; Base Sequence; Glycoside Hydrolases; Bacteroidetes; Malaysia; Galactose; Sequence Analysis, DNA; Seaweed; Agar; 03 medical and health sciences; Phosphoglucomutase; Energy Metabolism; Genome, Bacterial; Original Research

The agarolytic bacterium Persicobacter sp. CCB-QB2 was isolated from seaweed (genus Ulva) collected from a coastal area of Malaysia. Here, we report a high-quality draft genome sequence for QB2. The Rapid Annotation using Subsystem Technology (RAST) annotation server identified four β-agarases (PdAgaA, PdAgaB, PdAgaC, and PdAgaD) as well as galK, galE, and phosphoglucomutase, which are related to the Leloir pathway. Interestingly, QB2 exhibited a diauxic growth in the presence of two kinds of nutrients, such as tryptone and agar. In cells grown with agar, the profiles of agarase activity and growth rate were very similar. galK, galE, and phosphoglucomutase genes were highly expressed in the second growth phase of diauxic growth, indicating that QB2 cells use galactose hydrolyzed from agar by its agarases and exhibit nutrient prioritization. This is the first report describing diauxic growth for agarolytic bacteria. QB2 is a potential novel model organism for studying diauxic growth in environmental bacteria.