GOLPH3 promotes glioblastoma cell migration and invasion via the mTOR‐YB1 pathway in vitro

• DOI: 10.1002/mc.22197
• Publication Date: 2014-08-24T00:37:02Z
• Authors: Xu Zhang; Zhijun Ding; Jianbing Mo; Ben Sang; Qiong Shi; Jinxia Hu; Shao Xie; Wenjian Zhan; Dong Lu; Minglin Yang; Wenbin Bian; Xiuping Zhou; Rutong Yu
• Keywords: 0301 basic medicine; Brain Neoplasms; TOR Serine-Threonine Kinases; Down-Regulation; Membrane Proteins; 3. Good health; Up-Regulation; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Humans; Neoplasm Invasiveness; Y-Box-Binding Protein 1; Glioblastoma; Signal Transduction

The identification of genes involved in carcinogenesis and tumor progression is great interest, since these might be possible as candidates for new targeted therapy strategies. Our previous study shows that Golgi phosphoprotein 3 (GOLPH3) glioma cell migration invasion, the critical characteristics malignant gliomas. In this study, we explored mechanism GOLPH3 affecting invasion found promotes glioblastoma (GBM) via mammalian target rapamycin(mTOR)‐Y‐box binding protein‐1 (YB1) pathway vitro. Both protein levels YB1 were up‐regulated human tissues they exhibited direct correlation with each other. addition, down‐regulation inhibited while over‐expression enhanced them. Meanwhile, led to a significant decrease level well mTOR activity, both required invasion. On contrary, activity increased after over‐expression. or ATP site inhibitor INK128 treatment similar effect down‐regulation. Furthermore, induced was blocked by Taken together, results show mTOR‐YB1pathway, indicating GOLPH3‐mTOR‐YB1 therapeutic treatment. © 2014 Wiley Periodicals, Inc.