Bridging a 30 mm defect in the canine ulnar nerve using vessel‐containing conduits with implantation of bone marrow stromal cells

• DOI: 10.1002/micr.22391
• Publication Date: 2015-03-14T05:39:05Z
• Authors: Yukitoshi Kaizawa; Ryosuke Kakinoki; Ryosuke Ikeguchi; Souichi Ohta; Takashi Noguchi; Hiroki Oda; Shuichi Matsuda
• Keywords: Epineurial repair; Electrical conduit; Beagle

Previously, we showed that undifferentiated bone marrow stromal cell (uBMSC) implantation and vessel insertion into a nerve conduit facilitated peripheral regeneration in rodent model. In this study, investigated the efficacy of uBMSC-laden vessel-containing repair segmental defects, using canine Eight beagle dogs were used study. Thirty-millimeter ulnar defects repaired with conduits (right forelimbs, n = 8) or autografts (left 7). group, artery was inserted l-lactide/ε-caprolactone tube, which filled autologous uBMSCs obtained from ilium. autograft reversed segments sutured situ. At 8 weeks, one dog only sacrificed regenerated underwent immunohistochemistry for investigation differentiation capability implanted uBMSCs. remaining seven dogs, nerves electrophysiological examination at 12 24 weeks morphometric measurements weeks. The wet weight hypothenar muscles measured almost 35% expressed glial markers. amplitude (0.4 ± 0.4mV) conduction velocity (18.9 14.3m/s) significantly lower group than (3.2 2.5 mV, 34.9 12.1 m/s, P < 0.05). Although inferior when compared there no significant differences between both groups, regarding (10.9 7.3 vs. 25.3 20.1 mV; 0.11), (23.5 8.7 vs 31.6 20.0m/s; 0.35), myelinated axon number (7032 4188 7165 1814; 0.94), diameter (1.73 0.31 2.09 0.39μm; 0.09), muscle (1.02 0.40 1.19 0.26g; 0.36). conclusion, study tubes uBMSC may be an option treatment injuries. However, further investigations are needed. © 2015 Wiley Periodicals, Inc. Microsurgery 36:316-324, 2016.