Endometrial glycogen metabolism during early pregnancy in mice

• DOI: 10.1002/mrd.23634
• Publication Date: 2022-07-17T08:54:25Z
• Authors: Ziting Chen; Kassandra Sandoval; Matthew Dean
• Keywords: Decidualization; Periodic acid–Schiff stain; Immunostaining; Glycogen debranching enzyme

Abstract Glucose is critical during early pregnancy. The uterus can store glucose as glycogen but uterine metabolism poorly understood. This study analyzed storage and localization of metabolizing enzymes from proestrus until implantation in the murine uterus. Quantification diastase‐labile periodic acid–Schiff (PAS) staining showed glandular epithelium decreased 71.4% at 1.5 days postcoitum (DPC) 62.13% DPC 3.5 compared to proestrus. In luminal epithelium, was highest proestrus, 46.2% 63.2% 3.5. Immunostaining that before implantation, were primarily localized epithelium. Stromal low However, 5.5 sites, stromal levels increased sevenfold. Similarly, artificial decidualization resulted a fivefold increase levels. both models, expression synthase determine by immunohistochemistry western blot. conclusion, indicating it could be used support preimplantation embryos. Decidualization dramatic levels, suggesting may have an important, yet undefined, role