Metabolite profiling of the intraerythrocytic malaria parasitePlasmodium falciparumby1H NMR spectroscopy

• DOI: 10.1002/nbm.1323
• Publication Date: 2008-11-19T11:01:35Z
• Authors: Rongwei Teng; Pauline R. Junankar; William A. Bubb; Caroline Rae; Pascal Mercier; Kiaran Kirk
• Keywords: Perchloric acid; Phosphocholine; Hypoxanthine

NMR spectroscopy was used to identify and quantify compounds in extracts prepared from mature trophozoite-stage Plasmodium falciparum parasites isolated by saponin-permeabilisation of the host erythrocyte. One-dimensional (1)H four two-dimensional techniques were more than 50 metabolites. The intracellular concentrations over 40 metabolites estimated spectra extraction methods: perchloric acid, methanol/water, methanol/chloroform/water, methanol alone. quantified included: majority biological alpha-amino acids; 4-aminobutyric acid; mono-, di- tri-carboxylic nucleotides; polyamines; myo-inositol; phosphocholine phosphoethanolamine. also contained a significant concentration (up 12 mM) exogenous buffering agent, HEPES. Although metabolite profiles obtained with each method broadly similar, acid found have advantages other media.