Newborn cells in the adult crayfish brain differentiate into distinct neuronal types

• DOI: 10.1002/neu.20195
• Publication Date: 2005-08-19T19:47:24Z
• Authors: Jeremy M. Sullivan; Barbara S. Beltz
• Keywords: Cell type; Procambarus clarkii

Mitotically active regions persist in the brains of decapod crustaceans throughout their lifetimes, as they do many vertebrates. The most well-studied these decapods occurs within a soma cluster, known cluster 10, located deutocerebrum. Cluster 10 crayfish and lobsters is composed somata two anatomically functionally distinct classes projection neurons: olfactory lobe (OL) neurons accessory (AL) neurons. While adult-generated cells survive for at least year, final phenotypes remain unknown. To address this question, we combined BrdU labeling proliferating with specific neuronal glial markers tracers to examine differentiation newborn crayfish, Cherax destructor. Our results show that large numbers differentiate into expressing neuropeptide crustacean-SIFamide. No evidence was obtained suggesting glia. functional were examined by combining immunocytochemistry application dextran dyes different brain neuropils. These studies showed while majority born during early postembryonic development C. destructor AL neurons, neurogenesis adult characterized addition both OL In our examination pathway, provide first also characteristic feature optic neuropils crustaceans. © 2005 Wiley Periodicals, Inc. J Neurobiol,