An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells

• DOI: 10.1002/open.201900242
• Publication Date: 2019-10-07T12:17:47Z
• Authors: Yupeng Liu; Tian‐Bing Ren; Dan Cheng; Jianing Hou; Dongdong Su; Lin Yuan
• Keywords: Rhodamine

Abstract The novel ratiometric fluorescent probe HPQRB with an ESIPT effect based on Michael addition for highly sensitive and fast detection of sulfite in living HepG2 cells is reported. can be easily synthesized by a two‐step condensation reaction. has large emission shift (Δ λ =116 nm), which beneficial fluorescence imaging research, its sulfite‐responsive site rhodamine‐like structure the peak at 566 nm, decreases increasing concentration. HPQ always throughout reaction process, keeping 450 nm as self‐reference. In particular, high selectivity responds quickly (within 30 s) low limit (44 nM). Furthermore, been successfully used cells, demonstrating superior ability to detect under physiological conditions.