Combined analysis of T cell receptor γ and immunoglobulin heavy chain gene rearrangements at the single‐cell level in lymphomas with dual genotype

• DOI: 10.1002/path.1192
• Publication Date: 2002-10-09T03:09:42Z
• Authors: B. Vergier; P. Dubus; A. Kutschmar; M. Parrens; J. Ferrer; A. de Mascarel; J. P. Merlio
• Keywords: Immunoglobulin heavy chain

Abstract By prospectively studying immunoglobulin heavy chain gene ( IgH) and T cell receptor gamma TCR γ) rearrangements in 398 lymphoma cases, a dual genotype was observed 13% of B 11% lymphomas. According to histological subtype, the highest incidence for mantle lymphomas (32%) lymphoplasmacytic (21%) among lymphomas, angioimmunoblastic (AILT) (46%) Sézary syndrome (SS) (50%) To determine whether corresponds presence two distinct monoclonal populations or both within same cells, single‐cell microdissection used after immunohistochemistry combined IgH γ analysis designed whole‐genome amplification step. This protocol applied study nodal (one diffuse large one lymphoma) cutaneous AILT SS). Two cases (SS were true bigenotypic as detected cells. Conversely, CD22+ single cells exhibited only rearrangement but not that CD3+ Such an approach allows identification genotypic lymphoma. Specific genetic alterations may be further amplified from microdissected cryopreserved material, such t(11;14) breakpoint case. Copyright © 2002 John Wiley & Sons, Ltd.