Genome‐wide profiling of oral squamous cell carcinoma

• DOI: 10.1002/path.1640
• Publication Date: 2004-09-16T12:02:30Z
• Authors: Yann‐Jang Chen; Shu‐Chun Lin; Tsai Kao; Che‐Shoa Chang; Pei‐She Hong; Tzong‐Ming Shieh; Kuo‐Wei Chang
• Keywords: Profiling (computer programming)

Abstract Oral squamous cell carcinoma (OSCC) is a common malignancy, the incidence of which particularly high in some Asian countries due to geographically linked areca quid (AQ) chewing habit. In this study, array‐based comparative genomic hybridization was used screen microdissected OSCCs for genome‐wide alterations. The highest frequencies gene gain were detected TP63, Serpine1, FGF4/FGF3 , c‐ Myc and DMD. deletion Caspase8 MTAP . Gained genes, classified by hierarchical clustering, mainly on 17q21–tel; 20q; 11q13; 3q27–29 X chromosome. Among these, gains EGFR at 7p, FGF4/FGF3, CCND1 EMS1 11q13, AIB1 20q significantly associated with lymph node metastasis. profiles FHIT EXT1 AQ‐associated non‐AQ‐associated exhibited most prominent differences. RT‐PCR confirmed significant increase TP63 Serpine1 mRNA expression OSCC relative non‐malignant matched tissue. A immunoreactivity observed from tissue OSCC. However, there no correlation between frequent loss Caspase 8 decrease expression. These data demonstrate that profiling can be useful analysing pathogenetic events involved genesis or progression Copyright © 2004 Pathological Society Great Britain Ireland. Published John Wiley & Sons, Ltd.