Genome‐wide profiling of oral squamous cell carcinoma
Caspase 8
Fibroblast Growth Factor 3
Fibroblast Growth Factor 4
Genes, myc
Nucleic Acid Hybridization
DNA, Neoplasm
Middle Aged
Immunohistochemistry
Neoplasm Proteins
Fibroblast Growth Factors
03 medical and health sciences
0302 clinical medicine
Caspases
Lymphatic Metastasis
Proto-Oncogene Proteins
Plasminogen Activator Inhibitor 1
Carcinoma, Squamous Cell
Cluster Analysis
Humans
Mouth Neoplasms
RNA, Messenger
Oligonucleotide Array Sequence Analysis
DOI:
10.1002/path.1640
Publication Date:
2004-09-16T12:02:30Z
AUTHORS (7)
ABSTRACT
AbstractOral squamous cell carcinoma (OSCC) is a common malignancy, the incidence of which is particularly high in some Asian countries due to the geographically linked areca quid (AQ) chewing habit. In this study, array‐based comparative genomic hybridization was used to screen microdissected OSCCs for genome‐wide alterations. The highest frequencies of gene gain were detected for TP63, Serpine1, FGF4/FGF3, c‐Myc and DMD. The highest frequencies of deletion were detected for Caspase8 and MTAP. Gained genes, classified by hierarchical clustering, were mainly on 17q21–tel; 20q; 11q13; 3q27–29 and the X chromosome. Among these, gains of EGFR at 7p, FGF4/FGF3, CCND1 and EMS1 at 11q13, and AIB1 at 20q were significantly associated with lymph node metastasis. The genomic profiles of FHIT and EXT1 in AQ‐associated and non‐AQ‐associated OSCCs exhibited the most prominent differences. RT‐PCR confirmed the significant increase of TP63 and Serpine1 mRNA expression in OSCC relative to non‐malignant matched tissue. A significant increase in Serpine1 immunoreactivity was observed from non‐malignant matched tissue to OSCC. However, there was no correlation between the frequent genomic loss of Caspase 8 and a significant decrease in Caspase8 expression. These data demonstrate that genomic profiling can be useful in analysing pathogenetic events involved in the genesis or progression of OSCC. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
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