Histone H3 acetylation is associated with reduced p21WAF1/CIP1 expression by gastric carcinoma

• DOI: 10.1002/path.1684
• Publication Date: 2004-12-07T19:16:41Z
• Authors: Yoshitsugu Mitani; Naohide Oue; Yoichi Hamai; Phyu Phyu Aung; Shunji Matsumura; Hirofumi Nakayama; Nobuyuki Kamata; Wataru Yasui
• Keywords: Trichostatin A; Chromatin immunoprecipitation; Histone H4; SAP30; HDAC4; Histone deacetylase 5; HDAC11

Abstract Histone acetylation appears to play an important role in transcriptional regulation. Inactivation of chromatin by histone deacetylation is involved the repression several tumour suppressor genes, including p 21 WAF1/CIP1 . However, vivo status human cancers, gastric carcinoma, not well understood. This study shows that H3 WAF 1/ CIP 1 promoter region hypoacetylated and this hypoacetylation associated with reduced expression carcinoma specimens. Chromatin immunoprecipitation assays revealed was coding regions 10 (34.5%) 29 specimens, respectively. Hypoacetylation H4 observed 6 (20.7%) 16 (55.2%) mRNA levels were ( = 0.047) but 53 mutation 0.460). In cell lines, p21 protein induced trichostatin A, a deacetylase inhibitor. induction hyperacetylation region. Hyperacetylation did appear be increased expression. Induction histones Expression dominant‐negative mutant p53 protein. both gene cells expressing less than half wild‐type p53, whereas slightly (by approximately 20%) p53. These findings provide evidence alteration occurs cancer tissue specimens such as those from carcinoma. Copyright © 2004 Pathological Society Great Britain Ireland. Published John Wiley & Sons, Ltd.