Frequent alteration ofp16INK4a/p14ARF andp53 pathways in the round cell component of myxoid/round cell liposarcoma:p53 gene alterations and reduced p14ARF expression both correlate with poor prognosis

• DOI: 10.1002/path.1848
• Publication Date: 2005-09-21T12:46:32Z
• Authors: Yoshinao Oda; Hidetaka Yamamoto; Tomonari Takahira; Chikashi Kobayashi; Kenichi Kawaguchi; Naomi Tateishi; Yoko Nozuka; Sadafumi Tamiya; Kazuhiro Tanaka; Shuichi Matsuda; Ryohei Yokoyama; Yukihide Iwamoto; Masazumi Tsuneyoshi
• Keywords: p14arf

In myxoid/round cell liposarcoma (MLS/RCLS), the presence of a round (RC) component has been reported to correlate with worse prognosis for patients. However, little is known about molecular genetic differences between conventional myxoid (MX) components and RC in this tumour. The aim study was investigate possible implications alterations G1 S-phase check-point genes, especially component. We evaluated immunohistochemical expression p53, MDM2, p14 p16 protein assessed proliferative activities using MIB-1 29 81 MX from 90 cases. Mutation p53 gene, amplification MDM2 homozygous deletion, methylation status mutation p16(INK4a)/p14(ARF) genes were also investigated, concordant paraffin-embedded frozen material. data analysed together clinicopathological factors assess their prognostic MLS/RCLS. Immunohistochemically, over-expression (p = 0.01366) reduced < 0.0001) proteins significantly more frequently observed than components. Reduced correlated hypermethylation p14(ARF) gene promoter 0.0176) 0.00837). By univariate analysis, missense found reduce rate survival 0.05). Multivariate including factors, revealed that tumour site 0.0251), an 0.0113), high labelling index 0.0005) 0.0036) adverse factors. MLS/RCLS, reduction related poor prognosis. Accordingly, p14(ARF)/p53 pathway may contribute malignant progression