Uterine adenosarcomas are mesenchymal neoplasms

• DOI: 10.1002/path.4675
• Publication Date: 2015-11-23T09:07:05Z
• Authors: Salvatore Piscuoglio; Kathleen A Burke; Charlotte KY Ng; Anastasios D Papa...; Felipe C Geyer; Gabriel S Macedo; Luciano G Martelotto; Ino de Bruijn; Maria R De Filippo; Anne M Schultheis; Rafael A Ioris; Douglas A Levine; Robert A Soslow; Brian P Rubin; Jorge S Reis‐Filho; Britta Weigelt
• Keywords: Laser capture microdissection; Microdissection

Abstract Uterine adenosarcomas ( UAs ) are biphasic lesions composed of a malignant mesenchymal (ie stromal) component and an epithelial component. generally low‐grade have favourable prognosis, but may display sarcomatous overgrowth SO ), which is associated with worse outcome. We hypothesized that, akin to breast fibroepithelial lesions, neoplasms in clonal somatic genetic alterations restricted the To characterize test this hypothesis, we subjected 20 combination whole‐exome n = 6), targeted capture 13) massively parallel sequencing MPS and/or RNA 6) . Only three genes, FGFR2 , KMT2C DICER1 were recurrently mutated, all 2/19 cases; however, 26% (5/19) 21% (4/19) harboured MDM2 / CDK4 HMGA2 TERT gene amplification, respectively, two cases fusion genes involving NCOA family members. Using laser‐capture microdissection situ techniques, demonstrated that detected by Furthermore, mitochondrial DNA microdissected samples revealed components clonally unrelated. In conclusion, here provide evidence genetically heterogeneous neoplasms. Copyright © 2015 Pathological Society Great Britain Ireland. Published John Wiley & Sons, Ltd.