Clonality analysis of multifocal papillary thyroid carcinoma by using genetic profiles

• DOI: 10.1002/path.4696
• Publication Date: 2016-02-01T07:28:40Z
• Authors: Zheming Lu; Jindong Sheng; Yujie Zhang; Jianhua Deng; Yong Li; Aiping Lu; Juan Zhang; Huan Yu; Min Zhang; Zikai Xiong; Hai Yan; Bill H Diplas; Youyong Lu; Baoguo Liu
• Keywords: Field cancerization; Thyroid Nodules

Papillary thyroid carcinoma (PTC) is the most common adult malignancy and often presents with multiple anatomically distinct foci within thyroid, known as multifocal papillary (MPTC). The widespread application of next-generation sequencing technologies in cancer genomics research provides novel insights into determining clonal relationship between tumours same gland. For eight MPTC patients, we performed whole-exome targeted region to identify non-synonymous point mutations gene rearrangements spatially separated tumour foci. Among these MPTCs, completely discordant mutational spectra were observed cancerous nodules patients MPTC1 5, suggesting that originated from independent precursors. In another three cases (MPTC2, 6, 8), had no other shared except BRAF V600E, also indicating likely origins. Two (MPTC3 4) almost identical amongst their separate nodules, a origin. patient 7 seven foci, which two 66.7% mutations, while remaining displayed MPTC7 has origins accompanied by intraglandular disease dissemination. this study, found 75% arose tumours, supports field cancerization hypothesis describing malignant lesions. may arise intrathyroidal metastases single clone, well metastasis. Copyright © 2016 Pathological Society Great Britain Ireland. Published John Wiley & Sons, Ltd.