POU1F1 transcription factor promotes breast cancer metastasis via recruitment and polarization of macrophages

• DOI: 10.1002/path.5324
• Publication Date: 2019-07-11T07:43:15Z
• Authors: Samuel Seoane; Anxo Martinez‐Ord...; Noemi Eiro; Pablo Cabezas‐Sainz; Lucia Garcia‐Caba...; Luis O Gonzalez; Manuel Macia; Laura Sanchez; Francisco Vizoso; Roman Perez‐Ferna...
• Keywords: Intravasation; Extravasation; Tumor progression

Abstract Cancer progression requires cells surrounding tumors be reeducated and activated to support tumor growth. Oncogenic signals from malignant directly influence stromal composition activation, but the factors mediating this communication are still not well understood. We have previously shown that transcription factor POU class 1 homeobox (POU1F1), also known as Pit‐1, induces profound changes on neoplastic cell‐autonomous processes favoring metastasis in human breast cancer. Here we describe for first time Pit‐1‐mediated paracrine actions macrophages microenvironment by using cell lines vitro , zebrafish mouse models vivo samples cancer patients. Through release of CXCL12, Pit‐1 was found mediate recruitment polarization into tumor‐associated (TAMs). In turn, TAMs collaborated with increase growth, angiogenesis, extravasation lung. Our data reveal a new mechanism cooperation between poor clinical outcome cancer, which suggests CXCL12 should further studied potential prognostic therapeutic indicators. © 2019 Pathological Society Great Britain Ireland. Published John Wiley & Sons, Ltd.